Dr Derwood Pamphilon
Reader in Transfusion and Transplantation Medicine, Bristol Institute for Transfusion Sciences, National Blood Service, Southmead Road, Bristol
Honorary Consultant, Bristol Royal Hospital for Children, Bristol
Qualifications: MD, MRCPCH, FRCP, FRCPath
Research Interests: Development of strategies for the immunotherapy of relapsed acute lymphoblastic leukaemia (ALL). Our studies have focused on the growth of dendritic cells (DC) from patients with ALL at presentation or relapse. We have shown that functional dendritic cells may be obtained from children with ALL and used to stimulate the proliferation of allogeneic T-cells. Initially we studied the mixed lymphocyte reaction and the ability of T-cells to proliferate in response to presentation of CMV peptides by both normal and leukaemic dendritic cells. In initial experiments we showed that leukaemic DC could induce proliferation of T-cells with anti-leukaemic activity. Following Research and Ethics Committee approval to obtain more extensive cell samples of from children with ALL as well as their related and unrelated donors, we are aiming to further characterise the type of dendritic cells produced in these children, their functional characteristics and the nature of the T-cell response to them. If we are able to show that in a further cohort of number of children that we can reliably produce anti-leukaemic responses then we will apply to the Research and Ethics Committee for permission to undertake clinical studies. The approaches used in this study have much in common with those used to educate T-cells to eliminate viral infections in patients post-transplant.
Recent Publications:
Vettenranta K., Saarinen-Pihkala U.M., Cornish J., Steward C., Pamphilon D., Hovi L., Oakhill A. Pediatric marrow transplantation for acute leukemia using unrelated donors and T-replete or -depleted grafts: a case-matched analysis. Bone Marrow Transplantation 2000; 25; 395-399
Williams R.J., Clarke E., Blair A., Evely R., Hale G., Waldmann H., Brookes S., Pamphilon D.H. Impact on T-cell depletion and CD34 cell recovery using humanized CD52 monoclonal antibody (CAMPATH-1H) in BM and PBSC collections; comparison with CAMPATH-1M and CAMPATH-1G. Cytotherapy 2000; 2; 5-14
Rider J.R., Want E.J., Winter M.A., Turton J.R., Pamphilon D.H., Nobes P. Differential leucocyte subpopulation analysis of leucodepleted red cell products. Transfusion Medicine 2000; 10; 49-58
Murphy M.F., Pamphilon D.H., Devereux S. International Forum on Granulocyte Transfusions. Vox Sanguinis 2000; 79; 59-66
Marks D.I., Bird J.M., Vettenranta K., Hunt L., Green A., Cornish J.M., Goulden N., Pamphilon D.H., Steward C.G., Oakhill A. T cell-depleted unrelated donor bone marrow transplantation for acute myeloid leukaemia. Biology of Blood & Marrow Transplantation 2000; 6; 646-653
Baldwin A., Kingman H., Darville M., Foot A.B.M., Grier D., Cornish J.M, Goulden N., Oakhill A., Pamphilon D.H., Steward C.G, Marks D.I. Outcome and clinical course of 100 patients with adenovirus infection following bone marrow transplantation. Bone Marrow Transplantation 2000; 26; 1333-1338
Deeg H.J., Amylon I.D., Harris R.E., Collins R., Beatty P.G., Feig S., Ramsay N., Territo M., Khan S.P., Pamphilon D., Leis J.F., Burdach S., Anasetti C., Hackman R., Storer B., Mueller B. Marrow transplants from unrelated donors for patients with aplastic anemia: Minimum effective dose of total body irradiation. Biology of Blood and Marrow Transplantation 2001; 7; 208-215
Fuge R., Bird J.M., Fraser A., Hart D., Hunt L., Cornish J.M., Goulden N., Oakhill A., Pamphilon D.H., Steward C.G., Marks D.I. The clinical features, risk factors and outcome of thrombotic thrombocytopenic purpura occurring after bone marrow transplantation. British Journal of Haematology 2001; 113; 58-64
Vassiliou G.S., Webb D.K., Pamphilon D., Knapper S., Veys P.A. Improved outcome of alternative donor bone marrow transplantation in children with severe aplastic anaemia using a conditioning regimen containing low dose total body irradiation, cyclophosphamide and Campath. British Journal of Haematology 2001; 114; 701-705
Blair A., Rowbottom A.W., Browne S.J., Goulden N.J., Steward C.G., Oakhill A., Pamphilon D.H. An optimised biphasic culture system for the generation of functional dendritic cells from patients with acute lymphoblastic leukaemia at presentation and in clinical remission. Leukemia 2001; 15; 1596-1603
Blair A., Baker C.L., Pamphilon D.H., Judson P.A. Ex vivo expansion of megakaryocyte progenitor cells from normal bone marrow and peripheral blood and from patients with haematological malignancies. British Journal of Haematology 2002; 116; 912-919
Ronghe M.D., Foot A.B.M., Cornish J.M., Steward C.G., Carrington D., Goulden N., Marks D.I., Oakhill A, Pamphilon D.H. The impact of transfusion of leucodepleted platelet concentrates on cytomegalovirus disease after allogeneic stem cell transplantation. British Journal of Haematology 2002; 118; 1124-1127
Sohan K., Billington M., Pamphilon D., Goulden N., Kyle P. Normal growth and development following in utero diagnosis and treatment of homozygous a-thalassemia. British Journal of Obstetrics and Gynaecology 2002; 109; 1308-1310
Van Rhenen D., Gulliksson H., Cazenave J-P., Pamphilon D., Ljungman P., Klüter H., Vermeij H., Kapper-Klunne M., de Greef G., Laforet M., Lioure B., Davis K., Marblie S., Mayaudon V., Flament J., Conlan M., Lin L., Metzel P., Buchholz D, Corash L. Transfusion of pooled buffy coat platelet components prepared with photochemical pathogen inactivation treatment: the euroSPRITE trial. Blood 2003; 191; 2426-2433
Gilbert R.L., Rider J.R., Turton R., Pamphilon D.H. Detection of residual donor leucocytes in leucoreduced red blood cell components using a fluorescence microplate assay. Journal of Immunological Methods 2003; 274; 17-25
Chakrabarti S., Brown J., Guttridge M., Pamphilon D.H., Lankester A., Marks D.L. Early lymphocyte recovery is an important determinant of outcome following allogeneic transplantation with CD34 selected graft and limited T-cell addback. Bone Marrow Transplant 2003; July; 32(1); 23-30
Kerr J.P., Liakopoulou E., Brown J., Cornish J.M., Fleming D., Massey E., Oakhill A., Pamphilon D.H., Robinson S.P., Totem A., Valencia A.M., Marks D.I. The use of stimulated granulocyte transfusions to prevent recurrence of past severe infections after allogeneic stem cell transplantation. Br J Haematol, 2003; Oct; 123(1); 114-8
Chakrabarti S., Milligan D.W., Brown J., Osman H., Vipond I.B., Pamphilon D.H., Marks D.I. Influence of Cytomegalovirus (CMV) Seropositivity on CMV Infection, Lymphocyte Recovery and non-CMV Infections following T Cell Depleted Allogeneic Stem Cell Transplantation: A Comparison Between Two T Cell Depletion Regimes. Bone Marrow Transplant. 2004; Jan; 33(2): 197-204
Gibson B.E.S., Todd A., Roberts I., D.H. Pamphilon, Rodeck C., Bolton-Maggs P., Durbin G. British Committee for Standards in Haematology, Transfusion Task Force Writing Group. Transfusion Guidelines for Neonates and Older Children. 2004 British Journal of Haematology; 124;433 - 453.
Pamphilon D. Stem Cell Harvesting and Manipulation Vox Sanguinis July 2004;Volume 87, Supplement 1, Edinburgh 2004
Charlotte V Cox, Roger S Evely, Anthony Oakhill, Derwood H Pamphilon, Nicholas J Goulden, and Allison Blair Characterisation of Acute Lymphoblastic Leukaemia Progenitor Cells. Blood, November 2004, 104(9); 2919-2925
Steward CG, Blair A, Moppett J, Clarke E, Virgo P, Lankester A, Burger SR, Sauer MG, Flanagan AM, Pamphilon DH, Orchard PJ. High peripheral blood progenitor cell counts enable autologous backup before stem cell transplantation for malignant infantile osteopetrosis. Biol Blood Marrow Transplant. 2005 Feb;11(2):115-21.