GFP-tagged sorting nexin 1 (SNX1) is associated with a dynamic tubulo-vesicular early endosomal compartment. SNX1 is a member of the large sorting nexin family, members of which are believed to play a role on receptor sorting within the endosomal network. SNX1 contains a PX domain and a C-terminal BAR domain. The PX domain binds phosphoinositides, in particular phosphatidylinositol 3-monophosphate (PtdIns(3)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2), whereas the BAR domain senses membrane curvature. Together these distinct membrane-binding activities enrich SNX1 on the tubular elements of the early endosome. The movies show three HeLa cells expressing low levels of GFP-tagged SNX1, highlighting the dynamic formation of GFP-SNX1 positive membrane tubules. Frames were captured every second for a period of 50 seconds. This work is published in Carlton et al., (2004) Curr Biol 14, 1791-1800.
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