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Publication - Professor Christiane Berger-Schaffitzel

    Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor

    Citation

    Estrozi, LF, Boehringer, D, Shan, S-O, Ban, N & Schaffitzel, C, 2011, ‘Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor’. Nature Structural and Molecular Biology, vol 18., pp. 88-90

    Abstract

    We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.

    Full details in the University publications repository