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Publication - Dr Nicola Stevenson

    ER-to-Golgi trafficking of procollagen in the absence of large carriers

    Citation

    McCaughey, J, Stevenson, NL, Cross, S & Stephens, DJ, 2019, ‘ER-to-Golgi trafficking of procollagen in the absence of large carriers’. Journal of Cell Biology, vol 218., pp. 929-948

    Abstract

    Secretion and assembly of collagen are fundamental to the function of the extracellular matrix. Defects in the assembly of a collagen matrix lead to pathologies including fibrosis and osteogenesis imperfecta. Owing to the size of fibril-forming procollagen molecules it is assumed that they are transported from the endoplasmic reticulum to the Golgi in specialized large COPII-dependent carriers. Here, analyzing endogenous procollagen and a new engineered GFP-tagged form, we show that transport to the Golgi occurs in the absence of large (>350 nm) carriers. Large GFP-positive structures were observed occasionally, but these were nondynamic, are not COPII positive, and are labeled with markers of the ER. We propose a short-loop model of COPII-dependent ER-to-Golgi traffic that, while consistent with models of ERGIC-dependent expansion of COPII carriers, does not invoke long-range trafficking of large vesicular structures. Our findings provide an important insight into the process of procollagen trafficking and reveal a short-loop pathway from the ER to the Golgi, without the use of large carriers.

    Full details in the University publications repository