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Publication - Professor Paul Martin

    Drosophila immune cells extravasate from vessels to wounds using Tre1 GPCR and Rho signalling

    Citation

    Thuma, LJM, Carter, D, Weavers, H & Martin, P, 2018, ‘Drosophila immune cells extravasate from vessels to wounds using Tre1 GPCR and Rho signalling’. Journal of Cell Biology.

    Abstract

    Inflammation is pivotal to fight infection, clear debris and orchestrate repair of injured tissues. Although Drosophila have proven invaluable for studying extravascular recruitment of innate immune cells (hemocytes) to wounds, they have been somewhat neglected as viable models to investigate a key rate-limiting component of inflammation - that of immune cell extravasation across vessel walls - due to their open circulation. We have now identified a period during pupal development when wing hearts pulse hemolymph, including circulating hemocytes, through developing wing veins. Wounding near these vessels triggers local immune cell extravasation, enabling live imaging and correlative light-electron microscopy of these events in vivo. We show that RNAi knockdown of immune cell integrin blocks diapedesis, just as in vertebrates, and we uncover a novel role for Rho-like signalling through the GPCR Tre1, a gene previously implicated in the trans-epithelial migration of germ cells. We believe this new Drosophila model complements current murine models and provides new mechanistic insight into immune cell extravasation.

    Full details in the University publications repository