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Publication - Professor Pete Cullen

    The retomer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network

    Citation

    Wassmer, T, Attar, N, Harterink, M, van Weering, J, Traer, C, Oakley, J, Gould, B, Stephens, D, Verkade, P, Korswagen, H & Cullen, P, 2009, ‘The retomer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network’. Developmental Cell, vol 17 (1)., pp. 110 - 122

    Abstract

    Early endosome-to-trans-Golgi network (TGN) transport is organized by the retromer complex. Consisting of cargo-selective and membrane-bound subcomplexes, retromer coordinates sorting with membrane deformation and carrier formation. Here, we describe four mammalian retromers whose membrane-bound subcomplexes contain specific combinations of the sorting nexins (SNX), SNX1, SNX2, SNX5, and SNX6. We establish that retromer requires a dynamic spatial organization of the endosomal network, which is regulated through association of SNX5/SNX6 with the p150glued component of dynactin, an activator of the minus-end directed microtubule motor dynein; an association further defined through genetic studies in C. elegans. Finally,wealso establish that the spatial organization of the retromer pathway is mediated through the association of SNX1 with the proposed TGN-localized tether Rab6-interacting protein-1. These interactions describe fundamental steps in retromer-mediated transport and establish that the spatial organization of the retromer network is a critical element required for efficient retromer-mediated sorting.

    Full details in the University publications repository