University of Bristol
Proteomics Facility
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Related Literature
Technical Information
ThermoFisher Scientific Mass Spec. Handbook
http://www.piercenet.com/Files/1601927_MSHBv2_Intl.pdf
TMT approach
http://www.piercenet.com/previews/Vol12Iss03/1601715_PreviewsV12N3_pg25-27.pdf
2D Fluorescence Difference Gel Electrophoresis (2D-DIGE)
User Manual. Amersham Biosciences
http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/literature_intro
Select main product area Proteomics and sub-area Fluorescence difference 2D gel electrophoresis.
Selected Publications
- Evans VC, Barker G, Heesom KJ, Fan J, Bessant C, Matthews DA. (2012) Nature Methods 9, 1207-1211. De novo derivation of proteomes from transcriptomes for transcript and protein identification.
- Yuan W, Heesom K, Phillips R, Chen L, Trinder J and Lopez-Bernal A. (2012) Reproduction 144, 505-518. Low abundance plasma proteins in labour.
- Pellegrin S, Heesom KJ, van den Akker E, Daniels G and Toye AM. (2012) Plos ONE 7, e38356. Differential proteomic analysis of human erythroblasts undergoing apoptosis induced by Epo-withdrawal.
- Wu W, Teng M, Tran K, Heesom KJ, Matthews DA, Barr JN and Hiscox JA. (2012) J. Virol. 86, 7777-7789. The interactome of the human respiratory syncytial virus NS1 protein highlights multiple effects on host cell biology.
- Steinberg F, Heesom KJ, Bass MD, Cullen PJ. (2012) J. Cell. Biol. 197, 219-230. SNX17 protects integrins from degradations by sorting between lysosomal and recycling pathways.
- Jefferies R, Morganb E, Shawa S and Heesom K. (2011) Molecular and Biochemical Parasitology 180, 56-61. Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry.
- Barker EN, Darby AC, Helps CR, Peters IR, Heesom KJ, Arthur CJ, Crossett B, Hughes MA, Radford AD, and Tasker, S. (2011) Vet. Res. 42, 83-91. Molecular characterisation of the uncultivatable hemotropic bacterium Mycoplasma haemofelis.
- Bergmeier L, Heesom KJ, Babaahmady K, Kelly CG, Pido-Lopez J and Lehner T. (2010) Immunology 129, 506-515. Cytoskeletal proteins bound to hsp70 may elicit resistance to SIV infection of CD4+ T cells.
- Lam, YW, Evans VC, Heesom KJ, Lamond AI and Matthews DA. (2010). Molecular and Cellular Proteomics 9, 117-130. Proteomic Analysis of the nucleolus in adenovirus infected cells.
- Camats M, Kokolo M, Heesom KJ, Ladomery M. and Bach-Elias M. (2009). PLoS One 4, e8518. P19 H-Ras induces G1/S phase delay, maintaining cells in a reversible quiescence state.
- Kemp K, Hares K, Mallam E, Heesom KJ, Scolding N and Wilkins,A. (2009). J. Neurochem 114, 1569-1580. Mesenchymal stem cell-secreted superoxide dismutase promotes cerebellar neuronel survival.
- Bhahra G, Sood A, Fisher B, Cartwright L, Saunders M, Evans WH, Surpenant A, Lopez-Castejon G, Mann S, Davis SA, Hails LA, Ingham I, Verkade P, Lane J, Heesom K, Newson R and Case CP (2009) Nature Nanotechnology 4, 876-883. Nanoparticles can cause DNA damage across a cellular barrier.
- Richardson BM, Heesom KJ, Parsons SF, Anstee DJ and Frayne J. (2009) Proteomics – Clinical Applications 3, 1-12. Analysis of the differential proteome of human erythroblasts during in vitro erthyropoiesis by 2D DIGE.
- Van Helmond Z, Heesom KJ and Love S. (2009). J. Neuroscience Methods 176, 206-212. Characterisation of two antibodies to oligomeric Aβ and their use in ELISAs on human brain tissue homogenates.
- Clarke SJ, Khaliulin I, Das M, Parker JE, Heesom KJ and Halestrap AP. (2008). Circulation Research 102, 1082-1090. Inhibition of mitochondrial permeability transition pore opening by ischemic preconditioning is mediated by reduction of oxidative stress rather than mitochondrial protein phosphorylation.