University of Bristol

Proteomics Facility

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Related Literature
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Related Literature

Technical Information

ThermoFisher Scientific Mass Spec. Handbook

http://www.piercenet.com/Files/1601927_MSHBv2_Intl.pdf

TMT approach

http://www.piercenet.com/previews/Vol12Iss03/1601715_PreviewsV12N3_pg25-27.pdf

2D Fluorescence Difference Gel Electrophoresis (2D-DIGE)

User Manual. Amersham Biosciences
http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/literature_intro
Select main product area Proteomics and sub-area Fluorescence difference 2D gel electrophoresis.


Selected Publications

  1. Evans VC, Barker G, Heesom KJ, Fan J, Bessant C, Matthews DA. (2012) Nature Methods 9, 1207-1211. De novo derivation of proteomes from transcriptomes for transcript and protein identification.
  2. Yuan W, Heesom K, Phillips R, Chen L, Trinder J and Lopez-Bernal A. (2012) Reproduction 144, 505-518. Low abundance plasma proteins in labour.
  3. Pellegrin S, Heesom KJ, van den Akker E, Daniels G and Toye AM. (2012) Plos ONE 7, e38356. Differential proteomic analysis of human erythroblasts undergoing apoptosis induced by Epo-withdrawal.
  4. Wu W, Teng M, Tran K, Heesom KJ, Matthews DA, Barr JN and Hiscox JA. (2012) J. Virol. 86, 7777-7789. The interactome of the human respiratory syncytial virus NS1 protein highlights multiple effects on host cell biology.
  5. Steinberg F, Heesom KJ, Bass MD, Cullen PJ. (2012) J. Cell. Biol. 197, 219-230. SNX17 protects integrins from degradations by sorting between lysosomal and recycling pathways.
  6. Jefferies R, Morganb E, Shawa S and Heesom K. (2011) Molecular and Biochemical Parasitology 180, 56-61. Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry.
  7. Barker EN, Darby AC, Helps CR, Peters IR, Heesom KJ, Arthur CJ, Crossett B, Hughes MA, Radford AD, and Tasker, S. (2011) Vet. Res. 42, 83-91. Molecular characterisation of the uncultivatable hemotropic bacterium Mycoplasma haemofelis.
  8. Bergmeier L, Heesom KJ, Babaahmady K, Kelly CG, Pido-Lopez J and Lehner T. (2010) Immunology 129, 506-515. Cytoskeletal proteins bound to hsp70 may elicit resistance to SIV infection of CD4+ T cells.
  9. Lam, YW, Evans VC, Heesom KJ, Lamond AI and Matthews DA. (2010). Molecular and Cellular Proteomics 9, 117-130. Proteomic Analysis of the nucleolus in adenovirus infected cells.
  10. Camats M, Kokolo M, Heesom KJ, Ladomery M. and Bach-Elias M. (2009). PLoS One 4, e8518. P19 H-Ras induces G1/S phase delay, maintaining cells in a reversible quiescence state.
  11. Kemp K, Hares K, Mallam E, Heesom KJ, Scolding N and Wilkins,A. (2009). J. Neurochem 114, 1569-1580. Mesenchymal stem cell-secreted superoxide dismutase promotes cerebellar neuronel survival.
  12. Bhahra G, Sood A, Fisher B, Cartwright L, Saunders M, Evans WH, Surpenant A, Lopez-Castejon G, Mann S, Davis SA, Hails LA, Ingham I, Verkade P, Lane J, Heesom K, Newson R and Case CP (2009) Nature Nanotechnology 4, 876-883. Nanoparticles can cause DNA damage across a cellular barrier.
  13. Richardson BM, Heesom KJ, Parsons SF, Anstee DJ and Frayne J. (2009) Proteomics – Clinical Applications 3, 1-12. Analysis of the differential proteome of human erythroblasts during in vitro erthyropoiesis by 2D DIGE.
  14. Van Helmond Z, Heesom KJ and Love S. (2009). J. Neuroscience Methods 176, 206-212. Characterisation of two antibodies to oligomeric Aβ and their use in ELISAs on human brain tissue homogenates.
  15. Clarke SJ, Khaliulin I, Das M, Parker JE, Heesom KJ and Halestrap AP. (2008). Circulation Research 102, 1082-1090. Inhibition of mitochondrial permeability transition pore opening by ischemic preconditioning is mediated by reduction of oxidative stress rather than mitochondrial protein phosphorylation.

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