Membrane traffic pathways in mammalian cells
How do the tens of thousands of different proteins expressed in each individual mammalian cell end up in the right place within that cell? This is the central question that underlies the research going on in my lab.
Mammalian cells possess complex and dynamic intracellular membrane systems. Many integral membrane proteins move between different membranes within the cell in order to perform their function. My lab is interested in the movement of proteins, particularly integral membrane proteins, between the trans Golgi network (TGN; at the exit of the Golgi apparatus) and the cell surface. Several inter-related projects using techniques of molecular and cell biology as well as live cell imaging (using 'state of the art' facilities within the MRC funded Cell Imaging Facility) to address this area of protein trafficking.
Recent work has focused on the membrane protein tetherin (CD317). Tetherin is present in many cell types and has been shown to play multiple roles, from regulation of the actin cytsoskeleton in polarised epithelial cells to restriction of the release of a range of enveloped viruses (including HIV), see (http://www.bris.ac.uk/news/2009/6246.html) for more information.
Ruth Rollason, Pete Billcliff, Katie Dunstan and Rachel Curnock.
Bruce EA, Abbink TE, Wise HM, Rollason R, Galao RP, Banting G, Neil SJ, Digard P. (2012) Release of filamentous and spherical influenza A virus is not restricted by tetherin. Journal of General Virology. [Epub ahead of print] PMID: 22258861
Eva R, Bouyoucef-Cherchalli D, Patel K, Cullen PJ, Banting G. (2012) Inositol (1,4,5) trisphosphate 3-kinase A opposes NGF driven neurite outgrowth. PLoS ONE. 7(2): e32386.
Rollason R, Korolchuk V, Hamilton C, Jepson M, Banting, G. (2009) A CD317(tetherin)/RICH2 complex plays a critical role in the organisation of the sub-apical actin cytoskeleton in polarised epithelial cells. Journal of Cell Biology. 184: 721-736.
Goffinet C, Allespach I, Homann S, Tervo H-M, Habermann A, Rupp D, Oberbremer L, Kern C, Tibroni N, Welsch S, Krijnse-Locker J, Banting G, Kräusslich H-G, Fackler OT, Keppler OT. (2009) HIV-1 Antagonism of CD317 is Species-Specific and Involves Vpu-Mediated Proteasomal Degradation of the Restriction Factor. Cell Host & Microbe. 19: 285-297.