Preliminary Programme CLEM course, July 12 - 16 2010, Bristol:

This course is aimed at providing an overview of Correlative Light Electron Microscopy techniques for life science research. We try to achieve this by intertwining lectures with practical sessions. We would like to emphasise the interactive nature of the course and would invite participants to bring their questions and ideas and discuss them with us.

Short synopsis:

  • July 12 - 16 2010
  • Wolfson Bioimaging Facility, University of Bristol, Bristol, UK
  • Supported by UoB, Leica Microsystems, FEI company, and RMS
  • 10-12 participants, PhD students + Post docs, Life sciences
  • Course fee: £1200.- (this includes 4 nights of accommodation, lunch, and 2 dinners)

For additional info please send a mail to Dr. Paul Verkade: p.verkade@bristol.ac.uk

Preexisting knowledge: basic knowledge of light and electron microscopy (or more)

Lectures:

  • Principles of Light Microscopy
  • Principles of Electron Microscopy
  • Principles of Correlative Light Electron Microscopy
  • Markers and Probes
  • Photo conversion of GFP (GRAB)
  • Cryofluorescence
  • CLEM using High-pressure freezing
  • CLEM using Tokuyasu cryo immuno labeling
  • CLEM using pre-embedment labeling
  • Cryo CLEM
  • Integrated Light Electron Microscope
  • CLEM using Dual-beam SEM
  • Retracing tools

Practical sessions:

  • Cryo fluorescent stage
  • Cell locate dishes
  • CLEM using high-pressure freezing
  • Photoconversion (GRAB)
  • Pre-embedment labelling
  • Sample processing
  • Retracing

Instructors and lecturers:

  • Ed Brown, Mark Jepson, Judith Mantell, Paul Verkade: University of Bristol, UK
  • Linda van Driel: Leiden University Medical Center, NL
  • Roman Polishchuk, Elena Polishchuk, Alberto Luini: TIGEM, Napoli, I
  • Judith Klumperman: University Medical Center Utrecht, NL
  • John Robinson: Columbus, Ohio, USA
  • Julia Fernandez-Rodriguez:
  • Lucy Collinson: LRI, London, UK
  • Thomas Müller-Reichert: University Dresden, D
  • Alberto Diaspro, Katia Cortese, Genova, I

Download the poster

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