Equipment
Microscopes
| Tecnai 12 -FEI 120kV BioTwin SpiritTransmission
Electron Microscope (TEM)
Technical specifications |
This microscope is optimised to deliver
high contrast images from biological material. The system is very easy
to use and high quality electronic images are immediately available.Equipped
with FEI Eagle 4k x4k CCD camera. |
| Tecnai 20 -FEI 200kV Twin Lens Scanning
Transmission Electron Microscope (STEM)
Technical
specifications |
This microscope is optimised for cryo and
electon tomography TEM and STEM experiments as well as applications
requiring high resolution imaging or analysis of thick sections. The
cryo equipment includes rapid freezing equipment for preparing samples
and Gatan cryo holder, FEI cold box and Low Dose software for analysis
on the microscopeRoutinely fitted with LaB6 filament for highest resolution
results and a FEI Eagle 4k x4k CCD camera as well as plate camera. |
| Quanta 400FEI Scanning Electron
Microscope. (SEM)
Technical specifications |
Extremely easy to use and versatile SEM
offering standard high vacuum, low vacuum and environmental modes. Fitted
with Secondary and Back-scattered electron detectors and the option
of a peltier stage |
Additional Hardware
| Vitrobot |
FEI Vitrobot™ Mark IV, a fully automated
vitrification device for plunge-freezing of aqueous (colloidal) suspensions.
The frozen specimens can then be analysed in the microscope using a
Gatan CryoTransfer Specimen holder. |
| High Pressure Freezer |
Leica EMPACT2 + RTS. |
| Freeze Substitution |
Leica AFS2 + FSP Leica AFS |
| Cryoultramicrotome |
Leica EM UC6 with FC6 Cryo attachment |
| Ultramicrotome |
Reichert Ultracut S |
| Preparation Equipment |
Most sample preparation can be done in the Unit including
freeze drying, critical point drying and coating. |
| PC workstations |
Tomographic data can be analysed using FEI Inspect software
and then Amira ResolveRT software can be used for further 3d reconstructions
and visualization. |
Notes
For further information about our imaging systems or to arrange access to
any of the equipment or to arrange training, please contact p.verkade@bristol.ac.uk.
Users are reminded that images should not be left on hard disks. In the future
images will be networked but at the moment please ask if you need advice on
data removal and do not use USB devices unless they have been checked by us.
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