Equipment

Liquid Chromatography Mass Spectrometry

Orbitrap Fusion Trbrid Mass Spectrometer with ETD (Thermo Scientific)

Operated with up-stream Ultimate 3000 nano-LC system.

Ultra-high resolution and accurate mass instrument used for:

  • Protein identification from highly complex samples
  • SILAC and phospho-SILAC
  • TMT and phospho-TMT (using SPS-MS3 analysis)

LTQ-Orbitrap Velos Mass Spectrometer with ETD (Thermo Scientific)

Operated with up-stream Ultimate 3000 nano-LC system.

Ultra-high resolution and accurate mass instrument used for:

  • Protein interaction studies (AP-MS, IPs, etc.)
  • Protein identification from less complex samples

MALDI-Mass spectrometry

Bruker Daltonics UltrafleXtreme 2 (housed in the School of Chemistry Mass Spectrometry Facility)

High resolution and accurate mass instrument used for:

  • Protein identification from single gel bands

Automated Proteolytic Digestion

DigestPro MSi Robot (Intavis Bioanalytical Instruments)

Automates proteolytic digestion to minimise sample handling. 96-well plate format allows the digestion of multiple samples in a single run.


Liquid Chromatography

Ultimate 3000 Bio-RS HPLC system (Thermo Scientific)

Used for the fractionation of peptide mixtures using basic RP chromatography, SAX chromatography or SCX chromatography.

Orbitrap Fusion Trbrid Mass Spectrometer with ETD (Thermo Scientific)
LTQ-Orbitrap Velos Mass Spectrometer with ETD (Thermo Scientific)
DigestPro MSi Robot (Intavis Bioanalytical Instruments)
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