Selected Publications

Here are some examples of recent publications which have included work performed within the facility.

Protein Identification and Protein Interaction studies

  1. Maringer K, Yousuf A, Heesom KJ, Fan J, Lee D, Fernandez-Sesma A, Bessant C, Matthews DA, Davidson AD. (2017) BMC Genomics 18, 101-119. Proteomics informed by transcriptomics for characterising active transposable elements and genome annotation in Aedes aegypti.
  2. Battram AM, Durrant TN, Agbani EO, Heesom KJ, Paul DS, Piatt R, Poole AW, Cullen PJ, Bergmeier W, Moore SF, Hers I. (2016) J. Biol. Chem. 292, 1691-1704. The Phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3)-binder Rasa3 Regulates Phosphoinositide 3-kinase (PI 3-kinase)-dependent Integrin αIIbβ3 Outside-in Signaling.
  3. Jiménez-Castellanos JC, Wan Ahmad Kamil WN, Cheung CH, Tobin MS, Brown J, Isaac SG, Heesom KJ, Schneiders T, Avison MB. (2016) J Antimicrob. Chemother. 71, 1820-1825. Comparative effects of overproducing the AraC-type transcriptional regulators MarA, SoxS, RarA and RamA on antimicrobial drug susceptibility in Klebsiella pneumoniae.
  4. Denton RM, Pullen TJ, Armstrong CT, Heesom KJ, Rutter GA. (2016) Biochem. J. 473, 1165-1178.  Calcium-insensitive splice variants of mammalian E1 subunit of 2-oxoglutarate dehydrogenase complex with tissue-specific patterns of expression.
  5. Palumbo V, Pellacani C, Heesom KJ, Rogala KB, Deane CM, Mottier-Pavie V, Gatti M, Bonaccorsi S, Wakefield JG. (2015) Curr. Biol. 25. 1777-1783. Misato controls mitotic microtubule generation by stabilizing the tubulin chaperone protein-1 complex.
  6. Park JH, Szemes M, Vieira GC, Melegh Z, Malik S, Heesom KJ, Von Wallwitz-Freitas L, Greenhough A, Brown KW, Zheng YG, Catchpoole D, Deery MJ, Malik K. (2014) Mol. Oncol. 9, 617-627. Protein arginine methyltransferas 5 is a key regulator of the MYCN oncoprotein in neuroblastoma cells.
  7. Hamdollah Zadeh MA, Amin EM, Hoareau-Aveilla C, Domingo E, Symonds KE, Ye X, Heesom KJ, Salmon A, D'Silva O, Betteridge KB, Williams AC, Kerr DJ, Salmon AH, Oltean S, Midgley RS, Ladomery MR, Harper SJ, Varey AH, Bates DO. (2014) Mol. Oncol. 9, 167-178. Alternative splicing of TIA-1 in human colon cancer regulates VEGF isoform expression, angiogenesis, tumour growth and bevacizumab resistance.
  8. Hassona Y, Cirillo N, Heesom K, Parkinson EK, Prime SS. (2014) Br. J. Cancer 111, 1230-1237. Senescent cancer-associated fibroblasts secrete active MMP-2 that promotes keratinocyte dis-cohesion and invasion.
  9. Goggs R, Harper MT, Pope RJ, Savage JS, Williams CM, Mundell SJ, Heesom KJ, Bass M, Mellor H and Poole AW (2013) J. Biol. Chem. 288, 34217-34229. RhoG protein regulates platelet granule secretion and thrombus formation in mice.
  10. Bell AJ, Satchwell TJ, Heesom KJ, Hawley BR, Kupzig S, Hazell M, Mushens R, Herman A and Toye AM (2013) PLoS One 8, e60300. Protein distribution during human erythroblast enucleation in vitro.
  11. Pellegrin S, Heesom KJ, van den Akker E, Daniels G and Toye AM. (2012) PLoS One 7, e38356. Differential proteomic analysis of human erythroblasts undergoing apoptosis induced by Epo-withdrawal.
 

SILAC

  1. Emmott E, Sorgeloos F, Caddy SL, Vashist S, Sosnovtsev S, Lloyd R, Heesom K, Locker N, Goodfellow I. (2017) Mol. Cell. Proteomics 16 (4 suppl 1), S215-S229. Norovirus-mediated modification of the translational landscape via virus and host-induced cleavage of translation initiation factors.
  2. Kvainickas A, Orgaz AJ, Nägele H, Diedrich B, Heesom KJ, Dengjel J, Cullen PJ, Steinberg F. (2017) J. Cell. Sci. 130, 382-395. Retromer- and WASH-dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.
  3. McMillan KJ, Gallon M, Jellett AP, Clairfeuille T, Tilley FC, McGough I, Danson CM, Heesom KJ, Wilkinson KA, Collins BM, Cullen PJ. (2016) J. Cell. Biol. 214, 389-399. Atypical parkinsonism-associated retromer mutant alters endosomal sorting in specific cargo proteins.
  4. McCaughey J, Miller VJ, Stevenson NL, Brown AK, Budnik A, Heesom KJ, Alibhai D, Stephens DJ. (2016) Cell Rep.15,1648-1659. TFG promotes organization of transitional ER and efficient collagen secretion
  5. Fan J, Saha S, Barker G, Heesom KJ, Ghali F, Jones AR, Matthews DA, Bessant C. (2015) Mol. Cell. Proteomics 14, 3087-3093. Galaxy Integrated Omics: Web-based standards-compliant workflows for proteomics informed by transcriptomics
  6. Wynne JW, Shiell BJ, Marsh GA, Boyd V, Harper JA, Heesom KJ, Monaghan P, Zhou P, Payne J, Klein R, Todd S, Mok L, Green D, Bingham J, Tachedjian M, Baker ML, Matthews D, Wang LF. (2014) Genome Biol 15, 532. Proteomics informed by transcriptomics reveals Hendra virus sensitizes bat cells to TRAIL mediated apoptosis.
  7. McGough IJ, Steinberg F, Gallon M, Yatsu A, Ohbayashi N, Heesom KJ, Fukuda M, Cullen PJ. (2014) J. Cell. Sci. 127, 4940-4953. Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling.
  8. Alqahtani A, Heesom KJ, Bramson J, Curiel D, Ugai H, Matthews D. (2014) J. Gen. Virol. 95, 2504-2511. Analysis of purified wild type and mutant adenovirus particles by SILAC based quantitative proteomics.
  9. McGough IJ, Steinberg F, Jia D, Barbuti PA, McMillan KJ, Heesom KJ, Whone AL, Caldwell MA, Billadeau DD, Rosen MK, Cullen PJ (2014) Curr. Biol. 24, 1670-1676. Retromer binding to FAM21 and the WASH complex is perturbed by the Parkinson disease-linked VPS35(D620N) mutation.
  10. Chiu H-C, Hannemann H, Heesom KJ, Matthews D and Davidson A (2014) PLoS One 9, e93305. High-Throughput Quantitative Proteomic Analysis of Dengue Virus Type 2 Infected A549 Cells.
  11. Danson C, Brown E, Hemmings OJ, McGough IJ, Yarwood S, Heesom KJ, Carlton JC, Martin-Serrano J, May MT, Verkade P and Cullen PJ (2013) J. Cell Science 126, 4885-4899. SNX-15 links clathrin endocytosis to the PtdIns3P early endosome independently of the APPL1 endosome.
  12. Steinberg F, Gallon, M, Winfield M, Thomas E, Bell AJ, Heesom KJ, Tavare JM and Cullen PJ (2013) Nature Cell Biology 15, 461-471. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.
  13. Evans VC, Barker G, Heesom KJ, Fan J, Bessant C and Matthews DA. (2012) Nature Methods 9, 1207-1211. De novo derivation of proteomes from transcriptomes for evidence based transcript and protein identification.
  14. Wu W, Teng M, Tran K, Heesom KJ, Matthews DA, Barr JN and Hiscox JA. (2012) J. Virol. 86, 7777-7789. The interactome of the human respiratory syncytial virus NS1 protein interactome highlights multiple effects on host cell biology.
  15. Steinberg F, Heesom KJ, Bass MD, Cullen PJ. (2012) J. Cell. Biol. 197, 219-230. SNX17 protects integrins from degradations by sorting between lysosomal and recycling pathways.

TMT

  1. Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J. (2017) Nature Communications 8, 14750.  An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
  2. Wilson MC, Trakarnsanga K, Heesom KJ, Cogan N, Green C, Toye AM, Parsons SF, Ansteee DJ, Frayne J. (2016) Mol. Cell. Proteomics 15, 1938-1946.  Comparison of the proteome of adult and cord erythroid cells, and changes in the proteome following reticulocyte maturation.
  3. Zuccala ES, Satchwell TJ, Angrisano F, Tan YH, Wilson MC, Heesom KJ, Baum J. (2016) Sci. Rep. 6, 19766.  Quantitative phospho-proteomics reveals the Plasmodium merozoite triggers pre-invasion host kinase modification of the red cell cytoskeleton.
  4. Littlejohns B, Heesom KJ, Angelini GD, Suleiman MS. (2014) Clin. Proteomics 11, 34. The effect of disease on human cardiac protein expression profiles in paired samples from right and left ventricles.
  5. Trakarnsanga K, Wilson MC, Griffiths RE, Toye AM, Carpenter L, Heesom KJ, Parsons SF, Anstee DJ, Frayne J. (2014) PLoS One 9, e100874. Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS.
  6. Littlejohns B, Pasdois P, Duggan S, Bond AR, Heesom KJ, Jackson CL, Angelini GD, Halestrap AP, Suleiman MS. (2014) PLoS One 9, e100579. Hearts from mice fed a non-obesogenic high-fat diet exhibit changes in their oxidative state, calcium and mitochondria in parallel with increased susceptibility to reperfusion injury.
  7. Abdul-Ghani S, Heesom KJ, Angelini GD, Suleiman MS. (2014) Biomed. Res. Int. Volume 2014, Article ID 767812. Cardiac phosphoproteomics during remote ischemic preconditioning: a role for the sarcomeric Z-disk proteins.
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