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Publication - Professor Adam Perriman

    Designer artificial membrane binding proteins direct stem cells to the myocardium

    Citation

    Xiao, W, Green, TIP, Liang, X, Delint, RC, Perry, G, Roberts, MS, Le Vay, K, Back, C, Ascione, R, Wang, H, Race, P & Perriman, A, 2019, ‘Designer artificial membrane binding proteins direct stem cells to the myocardium’. Chemical Science.

    Abstract

    We present a new cell membrane modification methodology where the inherent heart tissue homing properties of the infectious bacteria Streptococcus gordonii are transferred to human stem cells. This is achieved via the rational design of a
    chimeric protein-polymer surfactant cell membrane binding construct, comprising the cardiac fibronectin (Fn) binding domain of the bacterial adhesin protein CshA fused to a supercharged protein. Significantly, the protein-polymer
    surfactant hybrid spontaneously inserts into the plasma membrane of stem cells without cytotoxicity, instilling the cells with a high affinity for immobilized fibronectin. Moreover, we show that this cell membrane reengineering approach
    significantly improves retention and homing of stem cells delivered either intracardially or intravenously to the myocardium in a mouse model.

    Full details in the University publications repository