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Publication - Professor Anthony Davis

    Targeted anion transporter delivery by coiled-coil driven membrane fusion

    Citation

    Mora, NL, Bahreman, A, Valkenier, H, Li, H, Sharp, TH, Sheppard, DN, Davis, AP & Kros, A, 2016, ‘Targeted anion transporter delivery by coiled-coil driven membrane fusion’. Chemical Science, vol 7., pp. 1768-1772

    Abstract

    Synthetic anion transporters (anionophores) have potential as biomedical research tools and therapeutics. However, the efficient and specific delivery of these highly lipophilic molecules to a target cell membrane is non-trivial. Here, we investigate the delivery of a powerful anionophore to artificial and cell membranes using a coiled-coil-based delivery system inspired by SNARE membrane fusion proteins. Incorporation of complementary lipopeptides into the lipid membranes of liposomes and cell-sized giant unilamellar vesicles (GUVs) facilitated the delivery of a powerful anionophore into GUVs, where its anion transport activity was monitored in real time by fluorescence microscopy. Similar results were achieved using live cells engineered to express a halide-sensitive fluorophore. We conclude that coiled-coil driven membrane fusion is a highly efficient system to deliver anionophores to target cell membranes.

    Full details in the University publications repository