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Professor Dek Woolfson

Professor Dek Woolfson

Professor Dek Woolfson
BA(Oxon), PhD(Cantab)

Professor of Chemistry and Biochemistry

Area of research

Protein design and its application in bionanotechnology and synthetic biology

Office Chem N410 Mons, Tue-Fri p.m. & Life Sciences 303 Tue-Fri a.m.
Cantock's Close,
Clifton, Bristol BS8 1TS
(See a map)

+44 (0) 117 39 41314
+44 (0) 117 954 6347

Summary

The primary basic research interest of the group is the informational aspect of the protein-folding problem; that is, how does the sequence of a protein determine its active, three-dimensional structure or fold?

We tackle this problem using the following multi-disciplinary approach:

  1. We use bioinformatics to garner sequence-to-structure relationships from protein sequence and structural databases.

  2. We test the relationships ("rules for protein folding") that we find in two ways: (a) through ab initio protein-structure prediction; and (b) via rational protein design, where we engineer natural protein structures, or design new ones completely from scratch (so-called de novo design).

  3. We then test our engineered and design proteins experimentally using biophysical methods. The peptides and proteins are made either by peptide synthesis, or via recombinant DNA methods and the expression of synthetic genes. The products and then characterised using methods including: solution-phase biophysics (CD, FT-IR and fluorescence spectroscopy, AUC and ITC); high-resolution structural biology (NMR spectroscopy and X-ray crystallography); and microscopy (EM, AFM and light microscopy).

  4. Finally, we explore potential applications of some the engineered and designed proteins in the burgeoning fields of bionanotechnology and synthetic biology.

Keywords

  • bionanotechnology
  • protein design
  • protein-folding problem
  • protein
  • bioinformatics
  • protein sequence
  • protein design

Memberships

Organisations

School of Chemistry

Chemistry staff

Research sections

Research areas and groups

Selected publications

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