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Publication - Professor Stephen Mann

    Photoswitchable Phase Separation and Oligonucleotide Trafficking in DNA Coacervate Microdroplets

    Citation

    Martin, N, Tian, L, Spencer, D, Coutable-Pennarun, A, Anderson, JL & Mann, S, 2019, ‘Photoswitchable Phase Separation and Oligonucleotide Trafficking in DNA Coacervate Microdroplets’. Angewandte Chemie - International Edition, vol 58., pp. 14594-14598

    Abstract

    Coacervate microdroplets produced by liquid–liquid phase separation have been used as synthetic protocells that mimic the dynamical organization of membrane-free organelles in living systems. Achieving spatiotemporal control over droplet condensation and disassembly remains challenging. Herein, we describe the formation and photoswitchable behavior of light-responsive coacervate droplets prepared from mixtures of double-stranded DNA and an azobenzene cation. The droplets disassemble and reassemble under UV and blue light, respectively, due to azobenzene trans/cis photoisomerisation. Sequestration and release of captured oligonucleotides follow the dynamics of phase separation such that light-activated transfer, mixing, hybridization, and trafficking of the oligonucleotides can be controlled in binary populations of the droplets. Our results open perspectives for the spatiotemporal control of DNA coacervates and provide a step towards the dynamic regulation of synthetic protocells.

    Full details in the University publications repository