Metabolomics

Metabolomics

Metabolomics provides high-throughput (up to 200 samples per day) quantitative NMR analysis of human serum and plasma samples. Quantified metabolomic measures are delivered per sample using a robust and well-established quantification platform. All samples received are stored prior to analysis in a dedicated biobanking facility with 24/7 monitoring. Experienced and professional staff handle the samples throughout, and are available to discuss requirements at all stages of the process.

The platform

We operate Nightingale Health’s NMR-based blood biomarker analysis platform. The platform provides over 220 quantified metabolomic measures per sample including 14 lipoprotein subclasses (with molecular information on cholesterol, triglyceride, and phospholipid concentration in each subclass), as well as fatty acids (e.g. ω-3 and ω-6 fatty acids) and other lipid species. Nightingale’s comprehensive biomarker analysis also measures numerous low molecular-weight metabolites (including amino acids, glycolysis related measures and ketone bodies), as well as measures of glycoprotein acetylation (GlycA) and apolipoproteins A-I and B.

The fully automated NMR-based biomarker quantification provides superior repeatability (CV%) compared to mass spectrometry methods and is coupled with certified quality control.

For more information on Nightingale’s platform and biomarker panel see: https://nightingalehealth.com, and the List of Quantified Metabolites (PDF, 523kB)

The experimental setup and epidemiological applications of the metabolic biomarker data have been reviewed here: American Journal of Epidemiology 2017 doi.org/10.1093/aje/kwx016

Sample requirements

  • Sample type: human serum or EDTA, Citrate and heparin plasma are accepted
  • Minimum volume: 100 μL or 350 μL
  • Written assurance of appropriate ethical approval/participant informed consent
  • Please enquire for other sample types.

The Facility

High-field NMR spectroscopy

Bruker Avance III HD 500MHz

  • Ascend™ 500 magnet operating at 11.7 Tesla (proton frequency: 500.13MHz)
  • Inverse triple resonance TXI probe with automated tuning/matching and Z-gradient coil
  • Avance III HD console with BSVT temperature control via a BCU I
  • SampleJet auto-sampler with cooled (6 °C) carousel and sample pre-heating

Bruker Avance III HD 600MHz

  • Ascend™ 600 magnet operating at 14.1 Tesla (proton frequency: 600.4MHz)
  • Nitrogen-cooled triple resonance probe (CryoProbe™ Prodigy TCI) with automated tuning/matching and Z-gradient coil
  • Avance III HD console with BSVT temperature control via a BCU I
  • SampleJet auto-sampler with cooled (6 °C) carousel and sample pre-heating

Fully automated sample preparation

  • Janus® Varispan liquid handling robot (PerkinElmer)
  • Eight channels, compatible with Bruker SampleJet NMR tube racks, 96-well plates and most standard microtubes

Contact

Mr Matt Goodwin
IEU Metabolomics Manager
MRC IEU Metabolomics Facility
Population Health Sciences
Bristol Medical School
University of Bristol
Oakfield House
Oakfield Grove
Bristol BS8 2BN

Tel: +44 (0)117 331 8095
Email: matt.goodwin@bristol.ac.uk

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