modelling membrane ion channel proteins
hERG voltage sensitive ion channel
solute effects on protein stability
Areas of expertise
My main research interest is membrane insertion of proteins and antibiotic peptides:
The insertion of polypeptides into membranes underlies many biological processes, including the assembly of membrane proteins, and the action of natural polypeptide toxins and some peptide antibiotics.
Depending on specific circumstances, adoption of hydrophobic or amphipathic helical structure on binding to the membrane surface may be followed by membrane insertion, resulting in transmembrane helical intermediates in a membrane protein folding pathway, the formation of stable or transient membrane ion channels or, in the case of some polypeptide toxins and antibiotics, the generation of a lytic pore.
We are using molecular biological and spectroscopic methods to characterise these processes in native, cell-free and model systems.