Directory of Experts

Details for Professor Stephen Halford

Personal details
Name Professor Stephen Halford
Job title Emeritus Professor
Department School of Biochemistry University of Bristol
Personal web page http://www.bris.ac.uk/biochemistry/research/seh.html
Contact details This expert can be contacted via the University of Bristol Public Relations Office.

To help us deal with your request, please mention the Directory of Experts when contacting the Public Relations Office.

work+44 (0)117 331 8092
email:
Qualifications BSc, PhD(Bristol), DSc, FRS
Professional details
Membership of professional bodies Fellow of Royal Society
Keywords endonuclease
restriction/modification
enzyme kinetics
DNA-protein interaction
DNA structure
Areas of expertise I focus on type II restriction endonucleases which require two copies of their recognition site in order to cleave DNA. These proteins are of interest as models for DNA-protein interactions and long-range communications between distant DNA sites. Below is an overview of the specific enzymes I study:
-Long rage communications: mechanisms by which restriction enzymes can mediate long range communications.
-SfiI: a tetramer which binds to and cleaves two sites concertedly. It is a type IIf endonuclease.
-SgrAI: a type IIf endonuclease. It exists as a monomer/dimer equilibrium in solution and is thought to tetramise on DNA.
-BspMI: a tetrameric type IIs endonuclease which binds to and cleaves two sites concertedly. It is unusual because most type IIs endonucleases are monomeric in solution.
-FokI: a type IIs endonuclease which binds to two sites but cleaves only one.
-BbvCI: a heterodimeric type II endonuclease which binds to an asymmetric recognition sequence.