Royal Microscopical Society Chair of the Cryo Microscopy Group
Areas of expertise
Our research focuses on understanding and visualising intracellular transport processes, both biosynthetic as well as endocytic. Cells have to make new proteins and transport these to the correct places for proper functioning of the cell. Likewise they have to do this for signals (proteins) from the outside environment. Cells have to interpret these signals and then route them correctly.
We mainly do this by combining light microscopy with electron microscopy. In these so-called Correlative Light Electron Microscopy experiments we make use of the advantages of doing live cell imaging at the light microscopy level and using the high spatial resolution of the electron microscope (Brown et al., 2009). In this way we can capture specific segregation and fusion events taking place inside the cell (Verkade, 2008).
The major project that is currently ongoing is to study the segregation of recycling and degradative cargo in the endocytic pathway.