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Publication - Dr Duncan Casey

    The grab-and-drop protocol

    A novel strategy for membrane protein isolation and reconstitution from single cells

    Citation

    Schrems, A, Phillips, J, Casey, D, Wylie, D, Novakova, M, Sleytr, UB, Klug, D, Neil, MA, Schuster, B & Ces, O, 2014, ‘The grab-and-drop protocol: A novel strategy for membrane protein isolation and reconstitution from single cells’. Analyst, vol 139., pp. 3296-3304

    Abstract

    We present a rapid and robust technique for the sampling of membrane-associated proteins from the surface of a single, live cell and their subsequent deposition onto a solid-supported lipid bilayer. As a proof of principle, this method has been used to extract green fluorescent protein (EGFP) labelled K-ras proteins located at the inner leaflet of the plasma membrane of colon carcinoma cells and to transfer them to an S-layer supported lipid bilayer system. The technique is non-destructive, meaning that both the cell and proteins are intact after the sampling operation, offering the potential for repeated measurements of the same cell of interest. This system provides the ideal tool for the investigation of cellular heterogeneity, as well as a platform for the investigation of rare cell types such as circulating tumour cells.

    Full details in the University publications repository