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Publication - Dr Duncan Casey

    A Novel, all-optical tool for controllable and non-destructive poration of cells with single-micron resolution

    Citation

    Casey, DR, Wylie, D, Gallo, J, Dent, MR, Salehi-Reyhani, A, Wilson, R, Brooks, NJ, Long, NJ, Willison, KR, Klug, DR, Neil, MAA, Neale, SL, Cooper, JM & Ces, O, 2015, ‘A Novel, all-optical tool for controllable and non-destructive poration of cells with single-micron resolution’. in: Bio-Optics: Design and Application, BODA 2015. Optical Society of America (OSA)

    Abstract

    We demonstrate controllable poration within ≈1 μm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates. This technique will allow new experiments in single-cell biology, particularly in neuroscience. As our understanding of the fundamental mechanistic processes underpinning biology expands, so does the need for high-precision tools to allow the dissection of the heterogeneity and stochastic processes that dominate at the single- and sub-cellular level. Here, we demonstrate a highly controllable and reproducible optical technique for inducing poration within specific regions of a target cell's plasma membrane, permitting localized delivery of payloads, depolarization and lysis experiments to be conducted in unprecedented detail. Experiments support a novel mechanism for the process, based upon a thermally-induced change triggered by the interactions of a near-IR laser with a biocompatible thin film substrate at powers substantially below that used in standard optoporation experiments.

    Full details in the University publications repository