Professor George Banting

Summary

Identification and characterisation of:

1. intramolecular signals involved in the faithful sorting/targeting/retention of integral membrane proteins specific to different intracellular organelles
2. the intracellular machinery required to recognise these signals.

This research involves techniques from the fields of both molecular and cell biology. Interest is focused on the Golgi apparatus, specifically the trans-Golgi Network (TGN). The TGN is a major site of intracellular protein sorting at the exit site from the Golgi stack.

We have particular interest in a specific integral membrane protein, TGN38, which cycles between the TGN and the cell surface. The regulated expression of wild type and mutated forms of this protein in stably transfected cells is providing us with much information concerning the mechanisms which govern membrane traffic in this part of the cell. We are in addition interested in the role(s) played by a specific membrane associated isoform of IP3 3-kinase and another protein (DAMP-1) which, like TGN38, cycles between the TGN and the cell surface.

We study the trafficking of TGN38, DAMP-1 and other proteins, using molecules tagged with the Green Fluorescent Protein (GFP) of the jellyfish A. victoria.

Activities / Findings

• Identification and characterisation of kinases associated with clathrin coated vesicles
• Studies on DAMP-1, a protein with a novel topology that traffcis between the TGN and the cell surface and that shares certain features with prion proteins
• Studies on the traffic of TGN38
• Identification and characterisation of small molecule inhibitors of membrane traffic pathways

Teaching

Lectures :-

• Biochemistry to First Year Vet students
• Biochemistry to second year Biochemistry students (honours and non-honours)
• Cell Biology to second year Biochemistry students (honours and non-honours)
• Molecular Genetics to second year students
• Final Year Biochemistry

Tutorials :-

• First Year Biochemistry students
• Second Year Biochemistry students
• First Year Medical students
• Second Year Molecular Genetics students

Keywords

• Golgi
• TGN
• lipids
• endocytosis
• secretion

Processes and functions

• Intracellular protein localisation

Methodologies

• Basic molecular and cell biology as well as biochemistry
• recombinant protein expression in bacterial and mammalian cells
• fixed and live cell imaging by confocal and wode-field microscopy

Memberships

Organisations

School of Biochemistry

Other sites

• Infection-immunity
• Neuroscience

Links

•   home page

Selected publications

• Rollason, R, Korolchuk, V, Hamilton, C, Jepson, M & Banting, G 2009, ‘A CD317/tetherin-RICH2 complex plays a critical role in the organization of the subapical actin cytoskeleton in polarized epithelial cells’. The Journal of Cell Biology, vol 184 (5)., pp. 721 - 736

Read more >

Recent publications

• Eva, R, Bouyoucef-cherchalli, D, Patel, K, Cullen, P & Banting, G 2012, ‘IP₃ 3-kinase opposes NGF driven neurite outgrowth’. PLoS One, vol 7(2)., pp. e32386 - e32386
• Bruce, E, Abbink, T, Wise, H, Rollason, R, Galao, R, Banting, G, Neil, S & Digard, P 2012, ‘Release of filamentous and spherical influenza A virus is not restricted by tetherin’. Journal of General Virology, vol 93(5)., pp. 963 - 969
• Goffinet, C, Allespach, I, Homann, S, Tervo, H, Habermann, A, Rupp, D, Oberbremer, L, Kern, C, Tibroni, N, Welsch, S, Krijnse-Locker, J, Banting, G, Kräusslich, H, Fackler, O & Keppler, O 2009, ‘HIV-1 Antagonism of CD317 is species specific and involves Vpu-mediated proteasomal degradation of the restriction factor’. Cell Host & Microbe, vol 5 (3)., pp. 285 - 297
• Sadasivan, S, Patil, A, Bromley, K, Hastie, P, Banting, G & Mann, S 2008, ‘Novel protein-inorganic nanoparticles prepared by inorganic replication of self-assembled clathrin cages and triskelia’. Soft Matter, vol 4., pp. 2054 - 2058
• Rollason, R, Korolchuk, V, Hamilton, C, Schu, P & Banting, G 2007, ‘Clathrin-mediated endocytosis of a lipid-raft-associated protein is mediated through a dual tyrosine motif’. Journal of Cell Science, vol 120 (21)., pp. 3850 - 3858
• Korolchuk, V, Cozier, G & Banting, G 2005, ‘Regulation of CK2 activity by phosphatidylinositol phosphates’. Journal of Biological Chemistry, vol 280 (49)., pp. 40796 - 40801
• Palmer, C, Lim, W, Hastie, P, Toward, M, Korolchuk, V, Burbidge, S, Banting, G, Collingridge, G, Isaac, J & Henley, J 2005, ‘Hippocalcin functions as a calcium sensor in hippocampal LTD’. Neuron, vol 47 (4)., pp. 487 - 494
• Pattni, K & Banting, G 2004, ‘Ins(1,4,5)P₃ metabolism and the family of IP₃-3kinases’. Cellular Signalling, vol 16., pp. 643 - 654
• Hascakova-Bartova, R, Pouillon, V, Dewaste, V, Moreau, C, Jacques, C, Banting, G, Schurmans, S & Erneux, C 2004, ‘Identification and subcellular distribution of endogenous Ins(1,4,5)P₃ 3-kinase B in mouse tissues’. Biochemical and Biophysical Research Communications, vol 323(3)., pp. 920 - 925

View complete publications list in the University of Bristol publications system

Networks & contacts

• Prof. Jeremy Henley
  Prof. Mike Tanner
  Dr. David Stephens
  Dr. Harry Mellor
  Prof. Pete Cullen
  many other collaborators
• both internally and externally