Unit name | General Laboratory Techniques |
---|---|
Unit code | SOCSM0008 |
Credit points | 10 |
Level of study | M/7 |
Teaching block(s) |
Academic Year (weeks 1 - 52) |
Unit director | Dr. Bond |
Open unit status | Not open |
Pre-requisites |
None |
Co-requisites |
None |
School/department | Bristol Medical School |
Faculty | Faculty of Health Sciences |
This unit will introduce theoretical aspects and practical details of laboratory methods that are highly relevant to translational cardiovascular medicine research. The following techniques will be discussed: analysis of gene function (via approaches such as siRNA gene silencing and synthetic inhibitors), gene transfer (plasmid and viral based), analysis of gene transcription (via qPCR), analysis of gene expression (via Western blotting and ELISA), immunocytochemistry and immunohistochemistry (light, fluorescent and confocal microscopy and quantification), cell analysis using FACS, analysis of cell proliferation, migration/invasion and apoptosis, purification and culture of several relevant cell types, detection of polymorphisms and SNPs. Apart from providing a basic introduction to laboratory research methods this unit also importantly aims to provide an overview of essential skills experimental design such as choice of negative and positive controls, and biological and experimental replicates.
Students successfully completing this module will be able to:
Coursework (contributing a total of 60% to the unit) consisting of:
Written exams (contributing a total of 40% to the unit) consisting of:
Western Blotting: B.D. Hames and D. Rickwood. Gel Electrophoresis of Proteins: A Practical Approach 3rd Edition, The Practical Approach Series, Oxford University Press, 1998.
Flow Cytometry : A Practical Approach, 3rd Edition. (Practical Approach Series). Edited by M.G. Ormerod. Oxford University Press (2000)
Cell Proliferation and Apoptosis - D Hughes and H Mehmet (Eds)
Microarray: Seo D, Ginsburg GS, Goldschmidt-Clermont PJ. Gene expression analysis of cardiovascular diseases: novel insights into biology and clinical applications. J Am Coll Cardiol. 2006 Jul 18;48(2):227-35.
Analysis of gene expression using qPCR: Stephen A. Bustin (2010) Why the need for qPCR publication guidelines?—The case for MIQE. Methods 50 (2010) 217–226
siRNA mediated gene silencing: Lars Aagaard, John J. Rossi (2007) RNAi therapeutics: Principles, prospects and challenges. Advanced Drug Delivery Reviews 59 (2007) 75–86.